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amino acid composition analysis中文是什么意思
- 中文翻譯
- 造句
- 氨基酸組成分析
- amino acid 氨基酸；氨酸。
- composition n. 1.作文(法)，作詩(法)，作曲(法)；作品，文章 ...
- analysis n. (pl. -ses ) 1.分解，分析；【數(shù)學】解 ...
- amino acid composition 氨基酸組成成分
- amino acid composition analyzer 氨基酸成分分析儀; 氨基酸組成分析儀
- seed amino acid composition 種子氨基酸組成
- the essential amino acid composition 主要氨基酸組成
- amino acid analysis 氨基酸分析
- amino acid sequence analysis 氨基酸序列分析; 胺基酸順序分析
- toof form aaa - amino acid analysis 分析技術之縮寫縮略語
- composition analysis 羽絨成分分析; 組成分析,成分分析; 作品分析
- acid, amino 氨基酸
- acid composition 酸液組成
- amino terminal analysis 氨基末端分析
- apparatus for composition analysis 成分分析儀器
- composition analysis instrument 成分分析儀器
- structure and composition analysis 結構成分分析
- amino acid and organic acid fermentation 氨基酸及有機酸發(fā)酵
- 6 amino acid injection 六合氨基酸注射液,肝醒靈注射液
- 6-amino acid injection 六合氨基酸注射液,肝醒靈注射液
- a amino acid transport system 氨基酸轉(zhuǎn)運系統(tǒng)
- abnormal amino acid sequence 異常氨基酸序列
- acetonegenic amino acid 生酮氨基酸
- acidic amino acid 酸性氨基酸
- action of essential amino acid 必需氨基酸作用
- It ' s pi is 5 . 2 as determined with ief . amino acid composition analysis showed that one subunit of 6 - phosphogluconate dehydrogenase has about 480 amino acids and there are plentiful of ala , asp , leu , ser , glu , thr , phe , lys in it
氨基酸組成分析表明：枯草芽孢桿菌6 -磷酸葡萄糖酸脫氫酶的亞基由約480個氨基酸殘基組成，富含丙氨酸、門冬氨酸、亮氨酸、絲氨酸、谷氨酸、蘇氨酸、苯丙氨酸、賴氨酸。 - The hwtx - i gene was chemically synthesized according to its known cdna sequence , the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor , the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis , then it was transformed into host strain gs115 , a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations , the multicopy transformant was named gh1 . gh1 was cultivated in flasks . after 6 days of induction by 0 . 5 % methanol , the supernatant was checked by 16 . 5 % tricine - sds page , which showed there was a band in the position of 3 . 5 - 6 . 1kd , then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column , after reverse phase hplc of ci8 and vacuum drying , the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page , maldi - tof mass spectrometry , amino acid composition analysis , the n - terminal amino acid sequence and its biological activity , the final field of the purified rhwtx - i was about 80mg / l , accounting for 23 . 6 % of it total secretory proteins
將帶有hwtx -基因的ppic9k經(jīng)blgii線性化后，轉(zhuǎn)化酵母宿主菌gs115原生質(zhì)體后經(jīng)篩選陽性克隆并經(jīng)表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉(zhuǎn)化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發(fā)酵上清經(jīng)90飽和度的( nh _ 4 ) _ 2so _ 4沉淀， yw - 3 ( mwc03000 )的超濾膜超濾，再經(jīng)cm陽離子交換， c _ ( 18 )反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經(jīng)質(zhì)譜鑒定，氨基酸組成分析和序列測定為正確的表達產(chǎn)物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。 - Was released , which was confirmed by molecular mass and amino acid composition analysis . ultraviolet and circular dichroism spectra were used to test the secondary structure of - mt - 3 . distinct from other - mt , - mt - 3 showed a negative trough at about 220nm in the cd spectrum , suggesting that an - helix might exist in its secondary structure
紫外吸收光譜及圓二色性光譜研究重組- mt - 3的結構。 mt - 3的與其余mt的結構域不同， - mt - 3的圓二色性光譜在220nm左右為負峰，說明- mt - 3可能包含一個-螺旋的二級結構。
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